IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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    Cucumber Gynogenesis: Effects of 8 Different Media on Embryo and Plant Formation

    Tugce Ozsan; Volkan Gozen; Ahmet Naci Onus
    Haploid and doubled haploid plants play a quite significant role in plant breeding programs. They allow obtaining pure lines from heterozygous parents in a short time. Present study was conducted to reveal the effects of different media on cucumber gynogenesis. The unfertilized cucumber ovaries of three genotypes (0701, 0702 and 0703) were collected one day before the anthesis. After surface sterilization, collected ovaries were sliced into 4 parts under sterile conditions, as pretreatment were placed on eight different modified cucumber basal media (CBM). All explants in different media were kept in dark conditions at 35°C for 3 days and then were transferred to 25°C in dark conditions for further 2 days. After the pretreatment at all cultured media were transferred to light conditions at 25°C in a photoperiod of 16/8 during 9 days. The effects of several factors such as genotypes and culture media were evaluated. And also callus, embryo formation and plantlets regeneration were recorded. Experiment results revealed that genotype 0703 and medium 5 (CBM with 0, 03 mg/L TDZ) combination gave the best results in terms of embryo formation and plantlet regeneration
    Select Volume / Issue:
    Year:
    2017
    Type of Publication:
    Article
    Keywords:
    Cucumis Sativus L; Cucumber; Gynogenesis; Haploidy
    Journal:
    IJAIR
    Volume:
    6
    Number:
    2
    Pages:
    419-422
    Month:
    September
    ISSN:
    2319-1473
    Hits: 2916

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