IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 15, Issue 1, July - Aug., 2026"
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Subsequent Development of Bovine Oocyte on In Vitro Maturation of Culture Media
- M. Saha; L. Asad
- The study was undertaken to find out the development of bovine oocyte supplementation by Bovine Serum Albumin (BSA) on in vitro maturation of culture media. Cumulus oocytes complexes (COCs) were collected from bovine ovaries by aspiration method and matured for 48 hours in TCM-199 basic medium supplemented with different levels of BSA (0%, 5%, 10% and 15%). The Significantly higher (p0.01) number of follicles were aspirated per ovary in ovaries without CL (4.60 ± 0.28) than with CL containing ovaries (3.00 ± 0.37). Higher numbers of COCs were found in ovaries without CL (4.42 ± 0.30) than ovaries with CL (2.40 ± 0.40). Higher number of follicles aspirated (p0.01) and the collected number of COCs in left ovaries (4.76 ± 0.20) and (4.47 ± 0.19 per ovary) respectively than right ovaries (4.74 ± 0.20) and (4.34 ± 0.18 per ovary) respectively. So, left ovary without CL is a good source of normal grade oocytes for in vitro maturation of bovine oocytes. The percentage of COCs reached to Metaphase-II stages with 0%, 5%, 10% and 15% of BSA supplementation were 0.00%, 0.00%, 0.12% and 0.00% respectively. These results further indicate that the maturation and subsequent development rate could be significantly increased (p0.01) by supplementing 5% level of BSA. This trend maintained up to 10% of BSA, but no further improvement was found by increasing level of BSA up to 15%. It is conducted that BSA has a positive effect on in vitro maturation of bovine oocytes and a 10% level of BSA is recommended based on the improvements observed and the associated economic benefits.
- Select Volume / Issue:
- Year:
- 2022
- Type of Publication:
- Article
- Keywords:
- Bovine Oocyte; Corpus Luteum; Bovine Serum Albumin; In-vitro Maturation
- Journal:
- IJAIR
- Volume:
- 10
- Number:
- 5
- Pages:
- 182-194
- Month:
- March
- ISSN:
- 2319-1473
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