IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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    Development of Efficient Suspension Formulation of Starch Industry Wastewater Grown Sinorhizobium Meliloti for Agricultural Use

    Rouissi Tarek; Tyagi Rajeshwar Dayal; Brar Satinder Kaur; Prevost Danielle
    Liquid and alfalfa seed coated formulations of Sinorhizobium meliloti were developed as potential bioinoculant. S. meliloti used in the present study was successfully cultivated using starch industry wastewater as the feedstock. The additives, such as sucrose, sorbitol, polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), sodium alginates, and different mixtures of these compounds were evaluated for their capacity to maintain the viability of S. meliloti during 13 weeks of refrigerated storage. All formulations were found to be capable of maintaining a viable cell count greater than 109 CFU/mL. Sucrose (10% w/v) was found to be the best additive to extend the half-life of the formulation to as long as 83 weeks. During 4 months of seed storage at ambient temperature, fresh liquid formulation of PEG and PEG-sorbitol applied to alfalfa seeds maintained the cell viability of 103 CFU/seed, which is equivalent to Canadian standards for commercial formulations. Micrographs showed that S. meliloti cells were distributed differently on the seed surface (as aggregates or uniformly distributed) according to the additives used. 13 week old PVP-sorbitol formulation applied on alfalfa seed was the most efficient formulation to facilitated highest shoot yield on dry biomass basis.
    Select Volume / Issue:
    Year:
    2015
    Type of Publication:
    Article
    Keywords:
    Sinorhizobium Meliloti; Formulation; Storage; Viability; Plant Yield
    Journal:
    IJAIR
    Volume:
    3
    Number:
    4
    Pages:
    1083-1093
    Month:
    Jan.-Feb.
    ISSN:
    2319-1473
    Hits: 5805

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