IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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    Effect of Duck Egg Yolk on Cryopreservation and Fertility of Egyptian Buffalo Bull Semen

    Mohamed E. El-Sharawy; El-Shenawy M. El-Seify; Ahmed M. Hussien; Mohamed El-Sherbieny; Ibrahim S. El-Shamaa
    The objective of this study was to compare the effectiveness of different duck egg yolk (DEY) concentrations (10, 15 and 20% DEY) with chicken egg yolk (20% CHEY) on the cryopreservation of Egyptian buffalo spermatozoa following dilution, equilibration and freezing-thawing processes. For this purpose, one ejaculate of semen from each of three Egyptian buffalo bulls was collected twice each week for 4 weeks with artificial vagina (42ºC). Pooled ejaculates were divided into four parts and were diluted in Tric citric acid glycerol extender containing either 10 or 15 or 20% DEY or 20% CHEY at 37ºC. Extended semen was equilibrated for 4h at 5ºC and then was filled in 0.5 ml straws and frozen in liquid nitrogen. Thawing of semen was performed at 37ºC for 30s. Progressive sperm motility, live sperm and plasma membrane integrity (%) after different stages of cryopreservation were assayed. The results of postthaw sperm motility for all extenders containing DEY showed significantly (P
    Select Volume / Issue:
    Year:
    2015
    Type of Publication:
    Article
    Keywords:
    Duck Egg Yolk; Cryopreservation; Buffalo Bull Semen; Fertility
    Journal:
    IJAIR
    Volume:
    3
    Number:
    4
    Pages:
    1133-1138
    Month:
    Jan.-Feb.
    ISSN:
    2319-1473
    Hits: 5837

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