IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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    Isolation, Screening and Characterization of Lactobacilli from Cow Milk

    R. B. Narwade; J. D. Kasare; R. S. Choudhary
    Lactobacillus species play a major role in fermented dairy products and also contribute to the therapeutic aspects of human health. Raw milk sample was used in this study to isolate and identify the lactobacillus and to find out the incidence of Lactobacillus in cow milk. Biochemical i.e. IMViC tests were used for identification of isolates of Lactobacillus from raw cow milk upto the genus level. From the Lactobacillus isolates, genomic DNA was extracted by using CTAB method. The size of the DNA was more than 20 Kb. The identification of Lactobacillus isolates by phenotypic methods is difficult. As it requires determination of bacterial properties beyond fermentation tests {&} time consuming therefore Polymerase Chain Reaction (PCR) – was used for identification of Lactobacillus at the genus level with genus specific primer. PCR showed positive reaction for isolates of Lactobacilli and amplicon size was 0.5 Kb.
    Select Volume / Issue:
    Year:
    2015
    Type of Publication:
    Article
    Keywords:
    Cow Milk; DNA isolation; IMViC Test; Lactobacilli; PCR
    Journal:
    IJAIR
    Volume:
    3
    Number:
    6
    Pages:
    1635-1637
    Month:
    May
    Hits: 4030

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