IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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    Proteomic Responses of Seed Embryo in Wheat Germination as Influenced by NaCl Stress

    Duan Jiang-Yan; Chen Yan-Yun; Wang Hui; Jia Zhen-Hu
    High extracellular NaCl can perturb and even kill cells, Many high-NaCl-induced perturbations responses are known. As protein metabolism plays an important role in plant growth, this study was designed to identify NaCl -responsive proteins in wheat seed. Wheat seed (Jinmai-47) was treated with NaCl at 0mol/L, 0.1mol/L, 0.2mol/L. Seed embryo proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE) in wheat germination, which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flightmass spectrometry, in which they were confirmed to be wheat seeds embryo proteins. The results showed: (1) NaCl at 0.1mol/L, 21 protein spots which could be identified were up-regulated and 32 protein spots were down-regulated. 3 new proteins were expressed. (2) NaCl at 0.2mol/L, 16 protein spots which could be identified were up-regulated and 46 protein spots were down-regulated. 2 new proteins were expressed. These data support the assumption that NaCl stress may have a regulatory role on protein metabolism of wheat seed and make it to regulatory protein expression to adapt the changed environment.
    Select Volume / Issue:
    Year:
    2015
    Type of Publication:
    Article
    Keywords:
    Wheat Embryo; NaCl Stress; Proteomic Responses
    Journal:
    IJAIR
    Volume:
    3
    Number:
    6
    Pages:
    1775-1779
    Month:
    May
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