IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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Studies on Ploidy analysis and chromosome doubling in Androgenic plants of Chilli pepper (Capsicum annuum L.)
- Arjunappa H. M; Sateesh Kumar. P; Prema Latha. D
- After successful production of chilli pepper (Capsicum annuum L.) androgenic plants through direct embryogenesis, the ploidy levels of the plants were checked by chromosome counting, chloroplast counting and flow cytometry methods. These results revealed that all the techniques were successful in assessing the ploidy levels. Besides chromosome count and chloroplast count techniques could be employed effectively as flow cytometry is expensive. The data obtained was that 12 chromosomes and 8-10 chloroplasts were observed in haploid plants, where as 24 chromosomes and 16-20 chloroplasts in double haploid plants. Plants which were haploids and those which did not undergo spontaneous doubling were treated with 0.5% colchicine and were tested for doubling.
- Select Volume / Issue:
- Year:
- 2016
- Type of Publication:
- Article
- Keywords:
- Chilli Pepper; Chromosome Doubling; Colchicine; Double Haploid; Flow Cytometry; Ploidy Analysis
- Journal:
- IJAIR
- Volume:
- 4
- Number:
- 4
- Pages:
- 627-633
- Month:
- January
- ISSN:
- 2319-1473
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