IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 15, Issue 1, July - Aug., 2026"
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 15, Issue 1, July - Aug., 2026"
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 15, Issue 1, July - Aug., 2026"
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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Studies Of Real-Time Rt-PCR Detection and Regular PCR for Phenotyping the Peach Old Varieties
- ION Ligia; MOREAU Fabienne; BADULESCU Liliana
- The TaqMan real time reversetranscriptaze (RT)- Polymerase chain reaction (PCR) using plant extract immobilized on a membrane (Whatman 3MM) to achieve targets for PPV is a procedure that has been developed to detect and quantify the virus Plum pox (PPV), in different varieties of peach . The sensitivity of the conventional real-time RT-PCR was 1,000 times higher than immunocapture (IC) -RT-PCR and 106-fold higher than the enzyme-linked immunosorbent assay (ELISA). The objective quantification of samples of PPV present in infected material which was then immobilized on the membrane Whatman 3MM can be used directly as a template without the need for a purification of RNA. In this study, different approaches were developed for sample preparation before PCR based Real Time Kit for detection TaqMan for a simple, rapidly, sensitive and universal quantification for PPV virus by real time RT PCR .Also the regular PCR was used to detect the PPV after artificial infection on the old Romanian varieties of peach („Novîi”, „Socini”,” Învingătorul”, „Oriol”, „De Voinești”,” Zalotnaia Oșeni”, „Miorița”, „Turist”, „Veteran”,” Cluj112” ).
- Select Volume / Issue:
- Year:
- 2016
- Type of Publication:
- Article
- Keywords:
- Varieties; Peach; Detection; Strains; Resistance; Plum Pox Virus
- Journal:
- IJAIR
- Volume:
- 4
- Number:
- 4
- Pages:
- 796-800
- Month:
- January
- ISSN:
- 2319-1473
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