IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 15, Issue 1, July - Aug., 2026"
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The Use of Bacillus Thuringiensis-based Products in Biocontrol of Tomato Leaf Miner, Tuta Absoluta (Lepidoptera, Gelechiidae)
- Afrooz AliRezaei; Reza Talaei-Hassanloui
- Tuta absoluta (Lepidoptera: Gelechiidae) is a micro-lepidopteran moth with high reproductive potential that attacks crop plants of the nightshade family, but tomato is the main host plant. Experiments have revealed some promising microbial agents for this moth including Bacillus thuringiensis (or Bt). Bt var. kurstaki has exhibited satisfactory efficacy against T. absoluta larval infestations in outbreaks. This bacterium produces protein crystals during sporulation which are toxic to insect larvae and cause rapid death of the host. According to this importance, efforts were made to estimate suitable time for using the Bt against T. absoluta on tomato. This moth was grown up on tomato plants under suitable temperature and humidity. In order to investigate the toxicity of two Bt strains on this pest, four concentrations were prepared and applied with two methods, leaf dipping and spraying. At the first method, Bt-treated tomato leaf discs were offered to larvae in different instars. At the second method, the bacterial suspensions were sprayed on leaf discs after the larvae were placed on them. For both methods and different ages, values LC50, LT50 and total mortality of larvae were estimated. Based on the results, there were no significant differences between LC50s of two bacterial strains for both leaf dipping and spraying methods. But the values of LT50 with the range of 37 to 61 hours were significantly different at all of larval stages. Comparison of mortality percentages for 2nd, 3rd and 4th instar larvae caused by 106 cells/ml after 24 hours revealed that death rate is significantly higher in the 4th instars than those in the early stages for both Bt application methods. Mortality rates of 2nd and 3rd larvae were affected by Bt application method and was higher significantly at the spraying method.
- Select Volume / Issue:
- Year:
- 2016
- Type of Publication:
- Article
- Keywords:
- Microbial Control; Bacillus Thuringiensis; Tuta Absoluta; Toxicity
- Journal:
- IJAIR
- Volume:
- 4
- Number:
- 4
- Pages:
- 814-817
- Month:
- January
- ISSN:
- 2319-1473
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