IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473
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Effect of Bovine Serum Albumin on In vitro Maturation, In vitro Fertilization and Subsequent Development of Goat Embryos
- L. Asad; M. A. M. Y. Khandoker; A. N. M. I. Rahman
- The present study was undertaken to find out the effect of bovine serum albumin (BSA) on In vitro maturation, fertilization and to support the subsequent embryonic development of goat oocytes. Cumulus oocytes complexes (COCs) were collected from slaughter house goat ovaries by aspiration method. COCs were matured for 24 hours in TCM-199 basic medium and supplemented with different levels of BSA (2mg/ml, 4mg/ml and 6mg/ml) where 0mg/ml was considered as control. Three levels of cumulus cell expansion after 24 h of In vitro culture (at 38.5ºC and 5% CO2 in an incubator) observed under 10x magnification of microscope and the different stages of nuclear maturation was observed based on chromosomal configuration. Metaphase-II stages were 40.78 ± 3.84, 67.52 ± 0.85, 68.95 ± 1.88 and 57.74 ± 2.39%. The fertility level was indicated by pronuclei formation. Normal fertilization (formation of 2 pronuclei) were 23.28 ± 3.00, 35.52 ± 1.21, 37.74 ± 1.24 and 29.30 ± 3.73% for 0 mg (control), 2 mg, 4 mg and 6 mg level of BSA respectively. The results indicated that the maturation and fertilization rate could be significantly increased (p0.01) by increasing the level to 4 mg per ml and when the BSA level was increased to 6 mg per ml, the rate of maturation and fertilization decreased significantly (p embryo development rate could be significantly (pd the rates could be improved further non-significantly by increasing the level to 4 mg/ml but significant (pon of BSA with TCM-199 is required for regular In vitro fertilization and the 2 mg per ml BSA might be used as a supplementation for In vitro maturation, fertilization and subsequent development of goat oocytes
- Select Volume / Issue:
- Year:
- 2017
- Type of Publication:
- Article
- Keywords:
- BSA; Goat Oocytes; In vitro Maturation; In vitro Fertilization; In vitro Culture
- Journal:
- IJAIR
- Volume:
- 5
- Number:
- 6
- Pages:
- 1096-1101
- Month:
- May
- ISSN:
- 2319-1473
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